Properties of Higher Plant Mitochondria. I. Isolation and Some Characteristics of Tightly-coupled Mitochondria from Dark-grown Mung Bean Hypocotyls
نویسندگان
چکیده
منابع مشابه
Properties of Higher Plant Mitochondria. I. Isolation and Some Characteristics of Tightly-coupled Mitochondria from Dark-grown Mung Bean Hypocotyls.
The mitochondria isolated from dark-grown mung bean hypocotyls oxidize succinate, l-malate, and externally added reduced nicotine adenine dinucleotide (NADH) with good respiratory control. While the pattern of respiration resembles that of animal mitochondria, there are 4 basic differences between the respiratory properties of mung bean and animal mitochondria: A) the ability to oxidize NADH, B...
متن کاملPreparation and some properties of submitochondrial particles from tightly coupled mung bean mitochondria.
Osmotic shock was found to be better than freezing and thawing, a French press, or sonic oscillation for the preparation of submitochondrial particles from mung bean (Phaseolus aureus) hypocotyl mitochondria. Particles prepared by osmotic shock rapidly oxidize reduced nicotinamide adenine dinucleotide and succinate, but they oxidize malate slowly. NADH oxidation was slightly stimulated by cytoc...
متن کاملNecessary conditions for isolation of tightly coupled higher plant mitochondria.
A number of parameters associated with the isolation of plant mitochondria were carefully examined in the hope of optimizing the isolation technique. Dark-grown mung bean (Phaseolus aureus var. Jumbo) hypocotyls were used as the source of mitochondria. Necessary conditions for the isolation of tightly coupled plant mitochondria include: (a) gentle tissue disruption; (b) rigorous exclusion of co...
متن کاملIsolation of tightly coupled mitochondria from acidic plant tissues.
T'b is comilitmication emphasizes the nieed for conltin1uouts conitrol of pH when macerating acidic plant tissues for the l)url)ose of isolating tightly coupled mitochondria. A useful device for such control is described. Mitochonidria capable of oxidative but not pl)osphorylative activity have been isolated fromli apple fruits wlhenl either alkaline homogeniates (5) or l)oIyvinylpyrrolidone (P...
متن کاملProperties of Higher Plant Mitochondria. II. Effects of DNP, m-Cl-CCP, and Oligomycin on Respiration of Mung Bean Mitochondria.
Effects of inhibitors of phosphorylation on the oxidation of succinate and of l-malate were investigated with tightly coupled mitochondria isolated from mung bean hypocotyls. When mitochondria were incubated with 2,4-dinitrophenol, or carbonyl cyanide m-chlorophenylhydrazone prior to the addition of substrate, the uncoupling effects of these chemicals were relatively small. This is probably cau...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 1967
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.42.1.67